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Normal HC11 and ras-transformed mouse mammary cells are resistant to the antiproliferative effects of retinoic acid BJMBR
Snitcovsky,I.; Katayama,M.L.H.; Folgueira,M.A.A.K.; Brentani,M.M..
The objective of the present study was to determine the effects of retinoic acid on the growth of the mouse mammary cells HC11 and HC11ras, which are a model for in vitro breast cancer progression. The expression of the two classes (RARs and RXRs) of retinoic acid receptor mRNAs was determined by Northern blot analysis. Receptor functional integrity was determined by testing whether RAR ß mRNA could be induced by retinoic acid. The effects of a 72-h exposure to 50 µM 13-cis retinoic acid on HC11 and HC11ras cell proliferation and HC11 cell differentiation were investigated by flow cytometric cell cycle analysis, and by determination of ß-casein mRNA expression, respectively. The possibility that retinoic acid would induce the expression of the vitamin D...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Retinoic acid receptors; Mouse mammary cells; HC11ras cells; Carcinogenesis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200019
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